"Net-based surveys are less subjective than direct observations but are limited regarding the area that can be sampled (net apertures 1–2 m and ships typically have to slow down to deploy nets, requiring dedicated ship's time). The plastic debris sampled is determined by net mesh size, with similar mesh sizes required to make meaningful comparisons among studies. Floating debris typically is sampled with a neuston or manta trawl net lined with 0.33 mm mesh. Given the very high level of spatial clumping in marine litter, large numbers of net tows are required to adequately characterize the average abundance of litter at sea. Long-term changes in plastic meso-litter have been reported using surface net tows: in the North Pacific Subtropical Gyre in 1999, plastic abundance was 335 000 items/km2 and 5.1 kg/km2, roughly an order of magnitude greater than samples collected in the 1980s. Similar dramatic increases in plastic debris have been reported off Japan. However, caution is needed in interpreting such findings, because of the problems of extreme spatial heterogeneity, and the need to compare samples from equivalent water masses, which is to say that, if an examination of the same parcel of water a week apart is conducted, an order of magnitude change in plastic concentration could be observed."