"The facts obtained in this study may possibly be sufficient proof of the causal relationship, that only the most sceptical can raise the objection that the discovered microorganism is not the cause but only an accompaniment of the disease [...] It is necessary to obtain a perfect proof to satisfy oneself that the parasite and the disease are [...] actually causally related, and that the parasite is the [...] direct cause of the disease. This can only be done by completely separating the parasite from the diseased organism [and] introducing the isolated parasite into healthy organisms and induce the disease anew with all its characteristic symptoms and properties."