"Oberth... began the book Men in Outer Space: New Projects for Rockets and Space Travel... written in the style accessible to the general reader. ...Published in 1955 in German, it was translated into English, French, Italian, Dutch, and even in Croatian! ...Oberth's new book was indeed "cosmic," and this gave it a cardinal distinction from his classic book of the 1920s. ...A supplemental chapter ...is devoted to "s," a theme which occupied Oberth all his life. A short description ...is already present in the 1923 book. In 1929, when he published his fundamental work, Ways to Space Travel, he included a much more comprehensive description... in the chapter... "Space Stations." ...[A]lmost the entire chapter is devoted to space mirrors. In the 1954 book... a new varient... is presented. Eventually in Bucharest in 1978, an entire book (in German) was devoted to this theme. ...[T]he primary purpose for the space mirror would today be called an ecological one ...At the time ...there was ...no robotics technology, and he assumed all the work after ...erection ...would ...be carried out manually by astronauts."

"Oberth proposed to shade planets located close to the sun... with gigantic cosmic shields... Vice versa, the planets... further from the sun... would be warmed... with... gigantic cosmic mirrors."

"[A] giant net (similar to a trawl) would be erected in outer space, constructed in a hexagonal mesh pattern. This net would be stretched out and a tension sufficient to rotate the entire net would be maintained by . The rotation would be begun by special rockets and... continue because it was in a vacuum. The diameter of each hexagon would be about 10 kilometers, and the entire mirror would have a circular disk shape with a diameter of about 100 to 200 kilometers. Within each hexagon, a round mirror approximately 10 kilometers in diameter, would be installed. ...[Each] single mirror would be capable of being [independently] tilted... initiated by... electric s."

"The glass may be launched to the shield location by a . A number of studies have indicated that mass drivers are feasible and economical for launching unmanned payloads from the lunar surface. If the glass sheet is sufficiently flexible it may be formed into sheet on the lunar surface and launched in rolls."

"Such a mirror, he asserted, could not only light up the cities at night... but could also have a decisive influence on weather and climate."

"[H]is belief... a reduction in the cost of building large structures in outer space could be achieved by delivering the necessary materials from the moon... [H]e presupposed the existence of the necessary industrial plants on the moon, but the end result would be a thousand-fold reduction in the cost of building large structures in space."

"A conceptually simple method for offsetting the greenhouse radiation trapping effects would be to decrease the solar heating by the use of a space-based solar shield."

"The time required for the removal of... [[w:Greenhouse gases|[greenhouse] gases]] from the atmosphere by natural processes is... uncertain: current estimates are several centuries. The uncertainties... [have] led to calls for... restrictions on the generation of greenhouse gases. ...The existence of a possible technical solution could... have a major short-term impact in influencing short term consumption restrictions, even if the solution could not be implemented until the next century."

"Approximately 2% of the solar radiation reaching Earth must be blocked to offset the predicted greenhouse trapping in the next century."

"Ref: Philander, Medizinische Märchen"

"Enough of this. They are only dreams of the future. Bold ones? Perhaps, but we have already experienced... bolder ideas. Who would have believed in 1894 that, a few years later, one would see through a person by means of Roentgen rays? PHILANDER's statement (Medical Fairy Tales), "Man will be made transparent like a jelly-fish", was bolder than this dream of the future; that required finding something completely new, while here we are dealing with laws of nature already known.—Accomplishing these things will certainly require the conversion of enormous energies. But were not hundred times greater sums of money expended during the World War? In one year, the nations of Europe spend more on smoking and drinking than the whole sodium reflector would cost. War and narcotics are quite unnecessary things, yet more money is spent on them than on something useful. Should not mankind, in an exceptional case, also save something for constructive work?"

"shields for planets such as Venus or Mars would... be large, complex structures requiring vast amounts of lunar or asteroidal material... and long-range transportation... One... stepping stone to understanding and mastering the technologies and processes... would be the construction of a shield to offset the greenhouse effect on... Earth. Such... would not require interplanetary capabilities."

"An ideal opaque shield would scatter the Earth bound solar energy into diffuse infrared energy."

"The shield may also be transparent and simply scatter the visible photons away from the Earth. ...A glass shield may act as a prism to deflect the sunlight away from the planet in accordance with ."

"The cultural tasks are... possible to fulfill. For example, if a sea route to the ports of Siberia is to be kept ice-free, a route must only be chosen that runs approximately in the direction of the winter wind from the Gulf Stream... light is thrown on a relatively narrow and short strip running from east to west... to the extent that the sky clouds over at this place. ...[T]he direction of the wind and ...the earth's rotation coincide. Hereby the earth always rotates as we need it ...By the time the light patch has passed along the whole stretch, the fog at the beginning will either have settled or been blown away... Then one can begin at the beginning again, Since the clouds hold the heat above the shipping lane... a reflector 100 km in diameter is... sufficient."

"can be formed into glass for either a transparent or opaque shield."

"In the north, on the other hand, outside of , there are no such masses of land-ice (however much ice there may be, no danger arises from melting ice that floats in the water), and the glaciers of Greenland will remain because if their high location and because there will be more snowfall on Greenland if the polar sea melts."

"The miniature facets are adjusted by hand... It is sufficient to simply let the sun shine on the miniature reflector and then turn the facets so that the reflected light strikes those parts of the globe corresponding to the region to be irradiated."

"Whether we must seek to constantly reflect the light on the earth vertically or are, in fact, able to is another question. I will assume that we can in order to study the single elements which determine the path of the reflector. (s are also used in calculations, although it is known that they do not exist.)"

"In the south... the main task of large reflectors... of making polar regions arable, is not feasible. If the glaciers of Antarctica were melted, the level of the ocean would rise uncomfortably (6-8 m). Hopefully, by then man will be sensible enough at least to leave a cold zone for the protection of nature."

"I could have restricted myself in this book to only the most sober physical calculations. But in order to create the necessary respect for my idea (otherwise a realization of this idea is unthinkable), I felt impelled to draw a few pictures of the future... and I have set up some fantastic claims. Naturally, here also, I have said nothing that might not be possible by present scientific standards, and I will now show that I am also on completely scientific ground with this idea of a reflector."

"A rocket with the necessary equipment is sent aloft and there given a lateral propulsion which puts it into an elliptical orbit around the earth. I will call this rotation about the earth "revolution". Major axis perpendicular to the ecliptic, perigee in the south 1,000 km above the earth's surface, apogee in the north 5,000 km above the earth's surface."

"So for the southern hemisphere and the tropics there would only remain the illumination of large cities at night and perhaps supplying solar plants with more light as well as the influencing of the weather."

"As in the rest of mathematical science, so in trigonometry, were the Arabs pupils of the Hindus […]"

"However, it is not unlikely that the Arabs, who received from the Indians the numeral figures (which the Greeks knew not), did from them also receive the use of them, and many profound speculations concerning them, which neither Latins nor Greeks know, till that now of late we have learned them from thence. From the Indians also they might learn their algebra, rather than from Diophantus."

"That he [Al-Khwarizmi] should have borrowed from Diophantus is not at all probable; … It is far more probable that the Arabs received their first knowledge of algebra from the Hindus, who furnished them with the decimal notation of numerals, and with various important points of mathematical and astronomical information."

"By comparison, the Sanskrit and Greek traditions were absorbed in a rather piecemeal fashion. In the one case there was a fragmentary rendering of Hindu literature and scientific works (channeled through Sind, until the Abbasids lost their grip on the province). Indian numerals, arithmetic, mathematics, philosophy and logic, mysticism, ethics, statecraft, military science, medicine, pharmacology, toxicology (works on snakes (sarpavidya) and poison (visavidya)), veterinary science, eroticism, astronomy, astrology and palmistry were transmit ted. Chess and chausar games were brought from India. We have a reference by an Arabic author from Andalusia to an Indian book on tunes and melodies. Indian fables and literary works are reflected in the Thousand and One Nights. Al-Biruni, before he came to India, had some Indian works in his library which were translated into Arabic under the early Abbasid caliph Al-Mansur (754-775) and the Barmakid vazirs of Harun ar-Rashid; amongst these were the Brahmasiddhanta or Sindhind and the Pahcatantra. When, in 1020, Al-Biruni began his study of Indian astronomy from the Sanskrit originals he was to find that the early works were still held in the same high esteem.13 To an appreciable extent, Sanskrit philosophy had already come to the attention of the Sasanid Persians and its influence in the Islamic world was sometimes mediated by Sasanid schools. ‘It was recognized among the Khusros (Akasira) of Persia that wisdom (hikma) originally came from al-Hind’.14 In Islam however Indian influences submerged under the tide of Greek and Hellenistic learning, falsafa and science, from the ninth century onwards."

"Much of the Hindu attitude and approach to mathematics was certainly conveyed to Western Europe through the Arabs. The algebraic methods formerly considered to have been invented by Al-Khowarizmi can now be seen to stem from Hindu sources."

"I have decided first to consider the majority of the authors who up to now have written about [algebra], so that I can fill in what they have missed out. They are very many, and among them Mohammed ibn Musa [Al-Khwarizmi], an Arab, is believed to be the first [...] I believe that the word “algebra” came from him, because some years ago, Brother Luca [Pacioli] of Borgo San Sepolcro of the Minorite order, having set himself the task of writing on this science, as much in Latin as in Italian, said that the word “algebra” was Arabic [...] and that the science came from the Arabs. Many who have written after him have believed and said likewise, but in recent years, a Greek work on this discipline has been discovered in the Library of our Lord in the Vatican, composed by a certain Diophantus of Alexandria, a Greek author [...] Antonio Maria Pazzi and I have translated five books (of the seven) [...] In this work we have found that he cites the Indian authors many times, and thus I have been made aware that this discipline belonged to the Indians before the Arabs."

"Integrins play an important role in cell adhesion by linking the cytoskeleton of cells to components in the extracellular matrix. In this capacity, integrins cooperate with different cell surface receptors, including growth factor receptors and G-protein coupled receptors, to regulate intracellular signaling pathways that control cell polarization, spreading, migration, survival, and gene expression. A distinct subfamily of molecules in the integrin family of adhesion receptors is formed by receptors that mediate cell adhesion to laminins, major components of the basement membrane that lie under clusters of cells or surround them, separating them from other cells and/or adjacent connective tissue. During the past decades, many studies have provided evidence for a role of laminin-binding integrins in tumorigenesis, and both tumor-promoting and suppressive activities have been identified."

"Laminins are composed of three polypeptide chains, designated as α, β, and γ. The C-terminal region of laminin heterotrimers, containing coiled-coil regions, short tails, and laminin globular (LG) domains, is necessary and sufficient for binding to integrins, which are the major laminin receptor class. Laminin recognition by integrins critically requires the α chain LG domains and a glutamic acid residue of the γ chain at the third position from the C-terminus. Furthermore, the C-terminal region of the β chain contains a short amino acid sequence that modulates laminin affinity for integrins. Thus, all three of the laminin chains act cooperatively to facilitate integrin binding. Mammals possess 5 α (α1–5), 3 β (β1–3), and 3 γ (γ1–3) chains, combinations of which give rise to 16 distinct laminin isoforms. Each isoform is expressed in a tissue-specific and developmental stage-specific manner, exerting its functions through binding of integrins."

"Laminin-α2-related congenital muscular dystrophy (LAMA2-CMD) is a devastating neuromuscular disease caused by mutations in the LAMA2 gene. These mutations result in the complete absence or truncated expression of the laminin-α2 chain. The α2-chain is a major component of the laminin-211 and laminin-221 isoforms, the predominant laminin isoforms in healthy adult skeletal muscle. Mutations in this chain result in progressive skeletal muscle degeneration as early as neonatally. Laminin-211/221 is a ligand for muscle cell receptors integrin-α7β1 and α-dystroglycan. LAMA2 mutations are correlated with integrin-α7β1 disruption in skeletal muscle."

"... We here postulate that basement membrane laminin is the key antigen in driving psoriasis, inducing a T cell-mediated autoimmune response. For laminin to be considered as the key autoantigen in psoriasis, it would be reasonable to expect the following to be demonstrable: (1) that autoantigens are present in psoriatic inflammation; (2) that basement membrane laminin is perturbed in involved and uninvolved skin, and that some of the pathological changes associated with psoriasis could be predicted as a sequel to this; (3) that disruption of the basement membrane is among the earliest events in the evolution of psoriatic lesions; (4) that as streptococcal pharyngitis is the most clearly defined event to trigger or exacerbate psoriasis, then a T cell-mediated autoimmune response to laminin should be anticipated as a potential sequelae to streptococcal pharyngitis; (5) that T cells in psoriasis can be shown to react to peptides with homology to laminin; (6) that HLACw6, as the most closely related gene associated with psoriasis and which is involved in antigen expression, should be preferentially expressed within lesional psoriasis towards the basement membrane, together with other proximal associated immune activity; and (7) that there is some association between antilaminin pemphigoid, a humorally mediated autoimmune disease to skin basement membrane laminin, and psoriasis."

"Laminins, heterotrimers of α, β, and γ chains, are prominent constituents of basal laminae (BLs) throughout the body. Previous studies have shown that laminins affect both myogenesis and synaptogenesis in skeletal muscle. Here we have studied the distribution of the 10 known laminin chains in muscle and peripheral nerve, and assayed the ability of several heterotrimers to affect the outgrowth of motor axons. ... we show that motor axons respond in distinct ways to different laminin heterotrimers: they grow freely between laminin 1 (α1β1γ1) and laminin 2, fail to cross from laminin 4 to laminin 1, and stop upon contacting laminin 11. The ability of laminin 11 to serve as a stop signal for growing axons explains, in part, axonal behaviors observed at developing and regenerating synapses in vivo."

"Adhesion of Aspergillus fumigatus, the causative agent of human aspergillosis, to the extracellular matrix protein laminin has been previously demonstrated. This study investigated the expression of laminin receptors during swelling of conidia, a step leading to germination and subsequent colonization of tissues. Scanning electron microscopy showed that the laminin binding sites were distributed over the external rodlet layer of resting conidia. During swelling, the characteristic rodlet layer progressively disintegrated and conidia surrounded by a smooth cell wall layer appeared. Flow cytometry using fluorescein isothiocyanate-conjugated laminin demonstrated that expression of laminin receptors at the surface of conidia was swelling dependent. Resting conidia expressed high levels of laminin receptors on their surface. A gradual decrease of laminin binding was then observed as swelling occurred, reaching a minimum for 4-h-swollen conidia. This correlated with a loss of adherence of swollen conidia to laminin immobilized on microtiter plates. Trypsin pretreatment of conidia reduced laminin binding. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and ligand blotting with laminin identified in a cell wall extract a major 72-kDa cell wall glycoprotein which binds laminin. Thus, one of the initial events in the host colonization may be the recognition of basement membrane laminin by this 72-kDa cell wall surface component."

"We have previously demonstrated that Staphylococcus aureus, a highly invasive bacteria, presents a 52-kDa surface protein that mediates its binding to laminin. In order to better characterize this receptor, we excised this putative laminin receptor from two-dimensional (2-D) PAGE and used it as antigen for raising a mouse hyperimmune serum which was for screening an S. aureus expression library. A single clone of 0.3 kb was obtained, and its sequence revealed 100% homology with S. aureus α-enolase. Moreover, amino acid sequencing of the 52-kDa protein eluted from the 2-D gel indicated its molecular homology with α−enolase, an enzyme that presents a high evolutionary conservation among species. In parallel, monoclonal antibodies raised against the S. aureus 52-kDa band also recognized yeast α-enolase in western blot analysis. These monoclonal antibodies were also able to promote capture of iodine-labeled bacteria when adsorbed to a solid phase, and this capture was inhibited by the addition of excess rabbit muscle α-enolase. Finally, the cell surface localization of S. aureus α-enolase was further confirmed by flow cytometry. Hence, α-enolase might play a critical role in the pathogenesis of S. aureus by allowing its adherence to laminin-containing extracellular matrix."

"Mutations of LAMB2 typically cause autosomal recessive Pierson syndrome, a disorder characterized by congenital nephrotic syndrome, ocular and neurologic abnormalities, but may occasionally be associated with milder or oligosymptomatic disease variants. LAMB2 encodes the basement membrane protein laminin β2 which is incorporated in specific heterotrimeric laminin isoforms and has an expression pattern corresponding to the pattern of organ manifestations in Pierson syndrome. Herein we review all previously reported and several novel LAMB2 mutations in relation to the associated phenotype in patients from 39 unrelated families. The majority of disease-causing LAMB2 mutations are truncating, consistent with the hypothesis that loss of laminin β2 function is the molecular basis of Pierson syndrome. While truncating mutations are distributed across the entire gene, missense mutations are clearly clustered in the N-terminal LN domain, which is important for intermolecular interactions. There is an association of missense mutations and small in frame deletions with a higher mean age at onset of renal disease and with absence of neurologic abnormalities, thus suggesting that at least some of these may represent hypomorphic alleles. Nevertheless, genotype alone does not appear to explain the full range of clinical variability, and therefore hitherto unidentified modifiers are likely to exist."

"Laminins are large molecular weight glycoproteins constituted by the assembly of three disulfide-linked polypeptides, the α, β and γ chains. The human genome encodes 11 genetically distinct laminin chains. Structurally, laminin chains differ by the number, size and organization of a few constitutive domains, endowing the various members of the laminin family with common and unique important functions. In particular, laminins are indispensable building blocks for cellular networks physically bridging the intracellular and extracellular compartments and relaying signals critical for cellular behavior, and for extracellular polymers determining the architecture and the physiology of basement membranes."

"The research on laminin α2 chain-deficient congenital muscular dystrophy (LAMA2-CMD) advanced rapidly in the last few decades, largely due to availability of good mouse models for the disease and a strong interest in preclinical studies from scientists all over the world. These mouse models continue to provide a solid platform for understanding the LAMA2-CMD pathology. In addition, they enable researchers to test laborious, necessary routines, but also the most creative scientific approaches in order to design therapy for this devastating disorder."

"Laminin-211 is a major constituent of the skeletal muscle basement membrane. It stabilizes skeletal muscle and influences signal transduction events from the myomatrix to the muscle cell. Mutations in the gene encoding the α2 chain of laminin-211 lead to congenital muscular dystrophy type 1A (MDC1A), a life-threatening disease characterized by severe hypotonia, progressive muscle weakness, and joint contractures. Common complications include severely impaired motor ability, respiratory failure, and feeding difficulties. Several adequate animal models for laminin-α2 chain deficiency exist and analyses of different MDC1A mouse models have led to a significant improvement in our understanding of MDC1A pathogenesis. Importantly, the animal models have been indispensable tools for the preclinical development of new therapeutic approaches for laminin-α2 chain deficiency, highlighting a number of important disease driving mechanisms that can be targeted by pharmacological approaches."

"The capacity of pathogenic microorganisms to adhere to host cells and avoid clearance by the host immune system is the initial and most decisive step leading to infections. Bacteria have developed different strategies to attach to diverse host surface structures. One important strategy is the adhesion to extracellular matrix (ECM) proteins (e.g., collagen, fibronectin, laminin) that are highly abundant in connective tissue and basement membranes. Gram-negative bacteria express variable outer membrane proteins (adhesins) to attach to the host and to initiate the process of infection. Understanding the underlying molecular mechanisms of bacterial adhesion is a prerequisite for targeting this interaction by “anti-ligands” to prevent colonization or infection of the host. Future development of such “anti-ligands” (specifically interfering with bacteria-host matrix interactions) might result in the development of a new class of anti-infective drugs for the therapy of infections caused by multidrug-resistant Gram-negative bacteria. This review summarizes our current knowledge about the manifold interactions of adhesins expressed by Gram-negative bacteria with ECM proteins and the use of this information for the generation of novel therapeutic antivirulence strategies."

"Extracellular matrix protein laminin binds specifically to yeast forms of Paracoccidioides brasiliensis and enhances adhesion of the fungus to the surface of epithelial Madin-Darby canine kidney cells in vitro. Immunoblotting of fungal extracts showed that the gp43 glycoprotein is responsible for adhesion. This was confirmed by binding assays using purified gp43, with a Kd of 3.7 nM. The coating of P. brasiliensis yeast forms with laminin before injection into hamster testicles enhanced the fungus virulence, resulting in a faster and more severe granulomatous disease. These results indicate that interaction of fungi with extracellular matrix elements may constitute a basis for the evolution of fungal infection toward regional spreading and dissemination."

"In adult rat testes, the basement membrane is structurally constituted by laminin and collagen chains that lay adjacent to the blood-testis barrier (BTB). It plays a crucial scaffolding role to support spermatogenesis. On the other hand, laminin-333 comprised of laminin-α3/ß3/γ3 at the apical ES (ectoplasmic specialization, a testis-specific cell-cell adherens junction at the Sertoli cell-step 8–19 spermatid interface) expressed by spermatids serves as a unique cell adhesion protein that forms an adhesion complex with α6ß1-integrin expressed by Sertoli cells to support spermiogenesis. Emerging evidence has shown that biologically active fragments are derived from basement membrane and apical ES laminin chains through proteolytic cleavage mediated by matrix metalloproteinase 9 (MMP9) and MMP2, respectively. Two of these laminin bioactive fragments: one from the basement membrane laminin-α2 chain called LG3/4/5-peptide, and one from the apical ES laminin-γ3 chain known as F5-peptide, are potent regulators that modify cell adhesion function at the Sertoli-spermatid interface (i.e., apical ES) but also at the Sertoli cell-cell interface designated basal ES at the blood-testis barrier (BTB) with contrasting effects. These findings not only highlight the physiological significance of these bioactive peptides that create a local regulatory network to support spermatogenesis, they also open a unique area of research."

"Anti-laminin antibodies were sought for in the serum of workers exposed to mercury vapour (Hg, n = 58), lead (Pb, n = 38) or cadmium (Cd, n = 47). Thirty-one workers removed from Cd exposure for an average of eight years were also examined. Compared with control workers matched for age and socio-economic status, the prevalence of circulating anti-laminin antibodies was not increased in workers exposed to Hg (mean duration of exposure: 7.9 years and mean urinary excretion of Hg: 72 μg/g creatinine) nor in those exposed to Pb (mean duration of exposure: 10.6 years and mean Pb levels in blood: 535 μg/l). In contrast, anti-laminin antibodies were significantly more prevalent in Cd-exposed workers whose urinary Cd exceeded 20 μg/g creatinine. This observation was made in both currently exposed workers and in workers removed from Cd exposure (mean duration of exposure: 9.4 and 24.6 years and mean urinary Cd: 7.8 and 13.4 μg/g creatinine respectively). These autoantibodies were found in Cd workers with normal renal function as well as in those with increased proteinuria."

"The major glycoprotein component of animal cell basement membranes, laminin, is involved in a variety of cellular activities, including cell adhesion, differentiation, and mito- genesis, that are mediated by the interaction of laminin with specific cell-surfacereceptors. A laminin-binding protein with an apparent molecular mass of 68 to 72 kD was first char- acterized in mammalian tumor cells and considered as “the laminin receptor” (Liotta et al., 1986; Wewer et al., 1986). Severa1 putative cDNA clones encoding this protein have been isolated from mammals (Yow et al., 1988; Rao et al., 1989; Van den Ouweland et al., 1989; Grosso et al., 1991). A11 the clones contained an open reading frame coding for a highly conserved polypeptide with a calculated molecular mass of 33 kD. Independently, a cDNA encoding an identical polypeptide was isolated from mouse tumor cells (Makrides et al., 1988), but the expressed protein, named factor p40, was shown to be a component of the translation machinery (Auth and Brawerman, 1992). Recently, DNA-deduced amino acid sequences exhibiting homology with the previ- ously characterized 33-kD ”laminin receptor” were identified from hydra (Keppel and Schaller, 1991), Drosophila (M.B. Melnick, T.B. Chou, and N. Perrimon, accession No. M90422), and yeast (J. Miles and T.G. Formosa, accession No. M88277)."

"Pseudomonas aeruginosa is a major human pathogen known to infect tissues that have been previously damaged in some way. In wounded human respiratory tissues, P. aeruginosa cells were found attached to exposed basement membranes following epithelial denudation, suggesting that the affinity for extracellular matrix proteins may account for the bacterium's opportunistic character. By using microtiter wells coated with different P. aeruginosa strains, we demonstrated that laminin binds to both colonizing bacterial strains, isolated from asymptomatic carriers, and strains isolated from infected patients. Binding of soluble laminin to piliated P. aeruginosa PAK and to the nonpiliated isogenic mutant PAK/p—was shown to be saturable. Binding of laminin to the piliated PAK strain was not different from binding to the nonpiliated PAK/p—strain but was significantly higher than binding to the avirulent, nonpiliated PAK-N1 rpoN mutant. By transmission electron microscopy, we localized the laminin-binding sites on a loose material in the outermost layer of the bacteria. Western immunoblotting results suggested that 57- and 59-kDa nonpilus adhesins from the microbial outer membranes account for the binding of P. aeruginosa to laminin. We speculate that bacterial affinity for laminin may be of biological significance in the pathogenesis of P. aeruginosa infection of injured tissues."

"Laminin, a non-collagenous glycoprotein present in the brain extracellular matrix, helps to maintain blood–brain barrier (BBB) integrity and regulation. Neuroinflammation can compromise laminin structure and function, increasing BBB permeability. ... We found that laminin may be a good indicator of BBB overall structural integrity, although changes in expression are dependent on the pathologic or experimental model used. In ischemic stroke, permanent vascular damage correlates with increased laminin expression (β and γ subunits), while transient damage correlates with reduced laminin expression (α subunits). Laminin was reduced in traumatic brain injury and cerebral hemorrhage studies but increased in multiple sclerosis and status epilepticus studies. Despite these observations, there is limited knowledge about the role played by different subunits or isoforms (such as 411 or 511) of laminin in maintaining structural architecture of the BBB under neuroinflammation."

"Retinal explants from embryonic or adult mice were placed on laminin or merosin substrates and the outgrowth of optic fibers was assayed under serum-free conditions. Both substrates strongly promoted outgrowth. A blocking antibody to the β1/β3 integrin subunits completely blocked laminin-dependent growth of embryonic optic fibers but had no detectable effect on adult fibers. Similarly, a blocking antibody against the main neurite-promoting region within the globular domain of the E8 fragment of laminin inhibited growth of embryonic fibers but had no effect on adult optic fibers. The β1 integrin subunit was identified immunohistochemically on both embryonic and adult fibers. These findings indicate that adult fibers have lost the β1 function which dominates laminin-dependent growth in embryonic fibers but express a receptor for laminin-dependent growth that is not detectable in embryonic fibers. These findings suggest that there are intrinsic differences between embryonic and adult optic fibers that may have implications for regenerative failure in the central nervous system of adult mammals."

"The normal microbial colonization of sites in the body's tissues by certain bacteria requires that the bacteria first bind to extracellular secreted constituents, cell-surface membranes, or cell matrixes. This study examines two interactions of a variety of bacteria with the cell matrix noncollagenous proteins fibronectin and laminin and with basement membrane (Type IV) collagen. Adherence of bacteria to matrix proteins coated on tissue culture wells was examined with the use of radiolabeled bacteria. Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus sanguis bound well to fibronectin, laminin, and Type IV collagen, whereas a variety of gram-negative organisms did not bind. The interaction of soluble laminin, fibronectin, and Type IV collagen with bacteria was monitored by nephelometry with the use of a platelet aggregometer. S. aureus aggregated in response to fibronectin, laminin, or Type IV collagen. In contrast, gram-negative organisms did not aggregate with these proteins. It appears that fibronectin, laminin, and Type IV collagen can bind and aggregate certain gram-positive bacteria, and this binding is dependent on the surface characteristics of the organism. These adhesion molecules may play a role in the normal colonization of sites by microorganisms and in invasion during infections."

"Blood vessels in the central nervous system (CNS) are unique in having high electrical resistance and low permeability, which creates a selective barrier protecting sensitive neural cells within the CNS from potentially harmful components in the blood. The molecular basis of this blood–brain barrier (BBB) is found at the level of endothelial adherens and tight junction protein complexes, extracellular matrix (ECM) components of the vascular basement membrane (BM), and the influence of adjacent pericytes and astrocyte endfeet. Current evidence supports the concept that instructive cues from the BBB ECM are not only important for the development and maturation of CNS blood vessels, but they are also essential for the maintenance of vascular stability and BBB integrity. In this review, we examine the contributions of one of the most abundant ECM proteins, laminin to BBB integrity, and summarize how genetic deletions of different laminin isoforms or their integrin receptors impact BBB development, maturation, and stability."