Angela N. H. Creager

"The 1940s and 1950s were marked by intense debates over the origin of drug resistance in microbes. ... Antibiotic resistance became a key issue among those disputing physiological (usually termed ‘’) vs. genetic ( and ) explanations of variation in . Postwar developments connected with the gave this debate a new political valence. Proponents of the weighed in with support for the genetic theory. However, certain features of drug resistance seemed inexplicable by mutation and selection, particularly the phenomenon of ‘multiple resistance’—the emergence of resistance in a single strain against several unrelated antibiotics. In the late 1950s, and his collaborators solved this puzzle by determining that resistance could be conferred by rather than . These could carry resistance to many antibiotics and seemed able to promote their own dissemination in bacterial populations. In the end, the vindication of the genetic view of drug resistance was accompanied by a recasting of the ‘gene’ to include extrachromosomal hereditary units carried on viruses and s."

"Laboratory instructions and recipes are sometimes edited into books with a wide circulation. Even in the late twentieth century, publications of this nature remained influential. For example, s from a 1980 summer course on at provided the basis for a bestselling laboratory manual by , and . Not only did the Molecular Cloning: A Laboratory Manual become a standard reference for s (commonly called the ‘bible’), but also its recipes and clear instructions made gene cloning and technologies accessible to non-specialists. Consequently, this laboratory manual contributed to the rapid spread of genetic-engineering techniques throughout the , as well as in industry. As is often the case with how-to books, however, finding a way to update methods in this rapidly changing field posed a challenge, and various molecular-biology reference books had different ways of dealing with knowledge obsolescence. This paper explores the origins of this manual, its publication history, its reception and its rivals – as well as the more recent migration of such laboratory manuals to the Internet."

"There are many reasons to revisit the history of research on (TMV), beginning with the fact that it was the first virus to be identified and so marks the start of the field of . However, not every original example of a new biological category becomes a well-studied object in its own right ... As virology took off in the early twentieth century, TMV did become one of the best-studied viruses and remained at the forefront of the field. It was used to elucidate basic knowledge about the nature of viruses and served as a in as well as agriculture, where it had emerged. The fact that the first recognized virus came from plants—although es were rapidly identified—meant that virology was, from the outset, highly comparative ... Literature on the origins of often privileges and the contributions of the ... Yet early work with TMV inspired Max Delbrück and other early molecular biologists to take up the study of bacteriophages. Moreover, TMV itself became a prominent model system for understanding the molecular nature of heredity and the relationship between proteins and nucleic acids ... Notably, some of the main scientists involved in elucidating the double-helical structure of DNA were also studying TMV, which became a tool for cracking the ."

"By 1950 the nature of the virus was no longer a mystery. Viruses were known to be s, genetic units, parasites that depend on their hosts for and . But a funny thing happened on the road to this knowledge. The viruses that most shaped this emerging portrait were not the most dangerous s, but those examples, however innocuous to humans, that made good laboratory subjects. Researchers constructed general knowledge about viruses based on a few that, by reason of historical precedence or biological robustness, were intensively studied as representatives of the rest."

"Like ’’’’, is part of the biologist's . The best-known model systems—standardized organisms such as the and the —are investigated by an entire community of biologists. Model systems become prototypes within which key biological questions are defined and resolved, useful precisely because they have already been so well studied. was a model system in these respects, studied and discussed by a large contingent of s, s, and other agricultural and medical researchers ..."

"s used s to reveal the sequence of chemical reactions in . s followed the assimilation and turnover of key s and tagged molecules such as to track the movement and activity of s. s labeled s with radioisotopes to follow the replication and expression of s. Physicians utilized radioisotopes such as and to diagnose and detect s. Ecologists profited as well, using to trace through the living and nonliving parts of aquatic and terrestrial landscapes, giving concrete meaning to the notion of an ecosystem."

"put to work in , having induced in an inbred mouse strain (Strong A) that was particularly susceptible to the implantation of tumors ... He and K. G. Scott found that these leukemic mice concentrated more radiophosphorus in their s and s than did healthy mice after both groups received tracer doses. ... This finding stoked hopes that radioisotopes would be selectively absorbed and localized in cancer patients, where they could serve to irradiate tumors."

"In Life Atomic, Angela Creager weaves an engaging tale of the history of s. Much of her material came from government documents from the Manhattan Project that were declassified during the . ... Creager introduces the concepts and vocabulary of radioisotopes at a level that any reader can appreciate."

"... one of the virtues of Creager's admirable book is that the attentive, even if scientifically uninformed, reader will learn a great deal, not only about these subjects but more generally about the character of during the last two-thirds of the twentieth-century. By tracking the history of from the applied realm of through its acceptance as —a widely and conventionally accepted laboratory tool—Creager traces more general trends in the development of , genetics, and . ... The power of Creager's method lies in how it underlines the dynamic set of relationships between ideas and experimental practice, between the laboratory and its sources of support."