"This marks the debut appearance of Leonardo, Raphael, Michelangelo and Donatello and their master and father Splinter, April O'Neil, her father Kirby, and of course The Shredder the leader of the Foot Clan."

"Karai is revealed to be the presumably dead biological daughter of Hamato Yoshi/Splinter and his late wife Tang Shen. Her true name being Miwa."

"Hamato Yoshi / Splinter - voiced by Hoon Lee"

"April O'Neil - voiced by Mae Whitman"

"Hamato Miwa/Karai - voiced by Kelly Hu"

"Oroku Saki/Shredder/Super Shredder/Zombie Shredder - voiced by Kevin Michael Richardson"

"Baxter Stockman / Stockman-Fly – voiced by Phil LaMarr"

"Kraang / Kraang Prime - voiced by Roseanne Barr"

"April O'Neil's psychic and extrasensory abilities, which were hinted in "Monkey Brains", are fully proven."

"Hamato Yoshi/Splinter"

"April O'Neil"

"Casey Jones (debut)"

"Oroku Saki/Shredder"

"Hamato Miwa/Karai (11 Episodes)"

"Dogpound/Razar"

"Baxter Stockman/Stockman Fly"

"Tiger Claw (debut; 7 Episodes)"

"Slash (debut; 2 Episodes)"

"Tang Shen (photographs/flashbacks; 8 Episodes)"

"Dr. Kirby O'Neil (7 Episodes)"

"Mrs. O'Neil (photograph; "The Kraang Conspiracy")"

"Timothy/the Pulverizer ("Mutagen Man Unleashed")"

"Dr. Falco/the Rat King ("Of Rats and Men")"

"Ivan Steranko"

"Anton Zeck"

"Leonardo/Leo"

"Raphael/Raph"

"Donatello/Donnie"

"Michelangelo/Mikey"

"April O'Neil"

"Casey Jones"

"Hamato Yoshi/Splinter (17 Episodes)"

"Hamato Miwa/Karai (10 Episodes)"

"Kirby O'Neil (6 Episodes)"

"Oroku Saki/Shredder"

"Takeshi/Tiger Claw"

"Xever/Fishface"

"Chris Bradford/Rahzar"

"Anton Zeck/Bebop"

"Ivan Steranko/Rocksteady"

"Baxter Stockman/Stockman Fly"

"Mrs. O'Neil ("Buried Secrets")"

"Tang Shen (pictures in 3 Episodes; "Tale of the Yokai")"

"The Mighty Mutanimals"

"This marks the debut appearance of Mona Lisa and Sal Commander."

"This marks the only appearance of the TMNT of the 1980s."

"Leonardo/Leo"

"Raphael/Raph"

"Donatello/Donnie"

"Michelangelo/Mikey/Michael"