"For these early results in Xenopus to be reproduced in mammals took nearly 40 years (Campbell et al. 1996; Wilmut et al. 1997) in sheep. A very important feature of these first successful mammalian nuclear transfer in sheep was the use of unfertilised eggs, as was actually used in amphibia. Earlier work with mice (McGrath and Solter 1984) used fertilised eggs. Although fertilised eggs can be used (Egli et al. 2007), synchronisation between nucleus and egg is harder to achieve than with the use of unfertilised eggs. A very elegant and important experiment that confirmed the general principle that cell differentiation proceeds with the retention of a complete set of genes was carried out using nuclei with a rearranged genome from mature mouse B or T donor cells (Hochedlinger and Jaenisch 2002). In the course of time, somatic cell nuclear transfer to eggs has been successful in the eggs of mice and other mammals (Wakayama et al. 1998). In each species there seem to be some technical requirements which have to be identified and overcome."
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University of Cambridge facultyNon-fiction authors from EnglandBiologists from EnglandNobel laureates in Physiology or MedicineNobel laureates from England
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(quote from pp. 238–239 of The Egg and the Nucleus)
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John Gurdon
Sir John Bertrand Gurdon (2 October 1933 – 7 October 2025) was an English zoologist, who shared the 2009 Lasker Award and shared the 2012 Nobel Prize for Physiology or Medicine with Shinya Yamanaka for stem cell research.
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